Thp1 assay

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WebApr 12, 2024 · An MTT assay was used to examine cell viability. Apoptosis was detected using FITC (fluorescein isothiocyanate) annexin V apoptosis detection kit. Western blotting was used to separate and identify proteins. ... Calcitriol also inhibited CCM-mediated induction of CD206 and increased TNFα gene expression in THP1-derived macrophages. WebAug 8, 2015 · After the incubation, the impact of etoposide on macrophage polarization was studied and cancer cell apoptosis was assessed by western-blot for cleaved caspase-3 …

Characterization of in vitro differentiated human macrophages. (A ...

Web9 hours ago · ß-lactamase assays were performed in THP1 infected cells as previously described 41. Around 10 5 THP-1 cells were seeded in a 96-well plate and differentiated for 72 hours using 50 µg/ml phorbol ... WebOct 27, 2024 · Kinase assay. WT THP1 cells were lysed in lysis buffer (1% (V/V) Nonidet P-40, 50 mM MOPS, pH 7.5, 5 mM MgCl 2, 0.5 mM MnCl 2, 100 mM NaCl, 0.4 mM Pefabloc SC, 0.1% (V/V) 2-mercaptoethanol) and ... bpr on property company

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WebTable 1. EC 50 values derived from a 4-parameter fit using SoftMax Pro Software.. Conclusion. We present a multiparametric inflammation assay that uses a THP-1 cell model with the ImageXpress Pico—an automated cellular imaging system—the Pu·MA System—a novel, low-volume automated ELISA system—and the SpectraMax iD5 microplate reader. WebAs a result of the WST-1 assay, the inhibitory concentration of diclofenac sodium at doses of IC 50 of 32.5 μM/mL and selonsertib was calculated to be 120 μM/mL. The immunological responses of diclofenac sodium and selonsertib (IL-1β) in different LPS-stimulated THP-1 cells were assessed cytometrically: diclofenac sodium and Selonsertib were found to be … WebDec 31, 2024 · For in vitro osteoclast differentiation assays, RAW264.7 cells were seeded at a density of 25,000 cells per well in 24‐well tissue culture ... NF‐κB reporter assay. THP1‐Dual cells were maintained in culture medium containing 10 µg/ml blasticidin and 100 µg/ml Zeocin to maintain selection pressure and passaged every three ... bpr on shares iht

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THP-1 cell line - Wikipedia

WebApr 14, 2024 · Separation between HUVECs and THP1 cells using flow cytometry would also depend on the intensity of the fluorescent dye used, so a dye with a high stain index is recommended for this assay. Also, a higher number of cells is necessary for flow cytometry than for microscopic evaluation in a 96-well plate, and this limitation could be significant … WebTHP1-Blue™ were derived from the human THP-1 monocyte cell line by stable integration of an NF-κB-inducible SEAP reporter construct. THP1- Blue™ NF-κB cells express a secreted … gynaecologist in albertonWebMar 27, 2014 · Typhimurium N-15 from a 6 h culture was diluted to 10 7 cfu/mL with RPMI with different Fe concentrations as described above in adhesion and invasion assays. THP1-Blue cells were seeded at approximately 10 5 cells/well in 96-well plates. 100 μL LPS-EK RPMI or RPMI containing S. gynaecologist in alberton prices

"WebFlow cytometric analysis showed that the common human monocytic cell lines (e.g., THP1 and U937) either do not express MerTK (THP1; data not shown) or express other efferocytosis receptors, such ... " - Thp1 assay

Thp1 assay

WebMar 3, 2024 · Moreover, Transwell assays revealed that IL-13RA2 overexpression mitigated KF migration and invasion (Figure 5, D and E). Western blot assay also showed that IL-13RA2 overexpression reduced the expression of mesenchymal markers (FN1, vimentin, and α-SMA), MMPs (MMP2 and MMP9), and collagen markers (COL1A1 and COL3A1) in KFs … WebApr 7, 2024 · 2.7. Cell Adhesion Assay. THP-1 monocytes were first activated by 20 ng/ml MCP-1 for 12 h. Then 2 × 10 5 THP-1 monocytes were added to each well containing a monolayer of confluent HUVECs in a 24-well tissue culture plate. THP-1 monocytes and HUVECs cells were incubated at 37°C in normoxia or IH with nonadhered cells removed by …

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WebThe contrast enhancement reduction on magnetic resonance imaging (MRI) of Mtb and THP1 cells showed proportional to the SPIO-MtbsAb nanoprobe concentration. After 30 min of intravenous SPIO-MtbsAb nanoprobe injection into Mtb-infected mice, the signal intensity of the granulomatous site was enhanced by 14-fold in the T2-weighted MR images … WebAug 7, 2008 · 160 nM PMA (Sigma) in RPMI medium was added to differentiate the THP-1 cells for 72 h at 37°C for all the cytokine assays through this study. The criteria for the differentiation of the THP-1 cells, adherence, morphological changes, and changes in expression of the cell surface makers such as integrin, FcγRI, CD4, and MHC class II …

WebJul 9, 2015 · The levels of (a) interleukin (IL)-2; (b) tumor necrosis factor (TNF)-α; (c) IL-10; and (d) IL-12 were measured in a THP1 cell line using a Bio-Plex Human Cytokine Th1/Th2 Assay. Particulate matter was collected in 2013 from 8 to 10 March, on 19 and 20 March, from 8 to 15 April, and from 30 April to 6 May. WebJan 13, 2024 · Allergen recognition and processing by antigen presenting cells is essential for the sensitization step of food allergy. Macrophages and dendritic cells are both phagocytic antigen presenting cells and play important roles in innate immune responses and signaling between the innate and adaptive immune system. To obtain a model …

WebTable 1. EC 50 values derived from a 4-parameter fit using SoftMax Pro Software.. Conclusion. We present a multiparametric inflammation assay that uses a THP-1 cell … WebTHP1 cells can be used in adhesion assays and transwell migration assay, ... For the migration assay, I re-suspend THP-1 cells in serum free RPMI media and seed 50,000 …

Web• Reporter activity has been validated using functional assays. • The stability for 20 passages following thawing has been verified. • THP1-Dual™ cells are guaranteed mycoplasma-free. USE RESTRICTIONS These cells are distributed for research purposes only. This product is covered by a Limited Use License. By use of this

WebTHP-1 cell line (ATCC TIB-202) is commonly used to study monocyte and macrophage activities, functions, innate immune mechanisms and signaling pathways. This luciferase reporter cell line was derived from parental line TIB-202 by stably expressing firefly luciferase gene (luc2) under control of a NF-κB promoter. This cell line was established … gynaecologist imagesWebDespite important impacts of systemic hypersensitivity induced by pharmaceuticals, for such endpoint no reliable preclinical approaches are available. We previously established … gynaecologist hialeah takes medicaidWebTHP-1. TIB-202 ™. THP-1 is a monocyte isolated from peripheral blood from an acute monocytic leukemia patient. This cell line can be used in immune system disorder … gynaecologist hyderabadWebMultiTox-Fluor Multiplex Cytotoxicity assays were performed using four increasing concentrations of DUBs-IN2 inhibitor in THP-1 macrophages as indicated on the figures. Cells were treated with either vehicle control or DUBs-IN2 for either a 2- or 24-hour period, after which the assay buffers were added and incubated for 30 minutes before … gynaecologist in bhiwandiWebOct 31, 2024 · Results from the two assays were significantly correlated (r = 0.70, p < 0.0001), with a higher percent phagocytosis found for many samples in the bead-based assay (Fig. 1c). b pro nursing agencyWebJun 21, 2024 · We used our previously established FACS-based phagocytosis assay 20 and performed a genome-wide CRISPR screen in THP-1 cells phagocytosing S. aureus particles. Subsequently, ... gynaecologist in carstenhof hospitalWebMethods: Cytotoxcity of WRE was determined at 24 and 72 h (h). Oxidant scavenging activity of WRE was evaluated (2, 2-diphenyl-1 picrylhydrazyl assay). Glutathione (GSH) levels, caspase (- 8, - 9, - 3/7) activities and adenosine triphosphate (ATP) levels (Luminometry) were thereafter assayed. gynaecologist in brenthurst hospital