Thp-1 cell atcc

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August undefined, 2024

WebDifferentiation in PMA-Stimulated THP-1 Cells and Monocyte-Derived Macrophages Marc Daigneault, Julie A. Preston, Helen M. Marriott, Moira K. B ... The THP1 cell line was obtained from ATCC, and maintained at 26105 cells/ml in RPMI 1640 medium supple-mented with 10% FCS and 2 mmol/L L-glutamine. THP1 cells (26105/ml) were differentiated using ... WebEBTr (bovine embryonic rainy winter weather. Nasal swabs were obtained from 109 tracheal) cells (ATCC CCL-44) and Calu-3 (human lung animals and 42 of these animals were positive for BCoV infection ... The THP-1 cells were maintained by control for infection of each cell line. either addition of freshly made growth medium or by ...

ATCC Announces New Immuno-oncology CAR-T Target Luciferase …

WebMaintaining healthy cells is the key to experimental success and reproducible research results. To give you confidence in the health of your cells every step of the way, we’ve … WebUsing membrane proteins extracted from human monocyte cell line THP-1 cells and a mixture of phospholipids, we formulated two types of PSF-extract-loaded biomimetic vesicles differing from each other for the presence of either 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) or 1,2-dioleoyl-sn-glycero-3-phospho-(1′-rac-glycerol) (DOPG). read zip file without extracting python

Journal of Community Medicine and Health Research

WebNF-kB-SEAP and IRF-Lucia luciferase Reporter Monocytes. THP1-Dual™ cells were derived from the human THP-1 monocyte cell line by stable integration of two inducible reporter constructs.. As a result, THP1-Dual™ cells allow the simultaneous study of the NF-κB pathway, by monitoring the activity of SEAP, and the IRF pathway, by assessing the … WebBiotransformation was done by three different strains of Lactobacillus plantarum (10, 8014, ATCC). Results demonstrated the importance of used solvent/treatment for extraction procedure and strains of L. plantrum. The lowest values of main allergens were determined as 321 ng/mL for BCAFE, 320 ng/mL for 1.1 DMAECAFE and 8.02 ng/mL for CAPE. WebAug 8, 2015 · Cell culture. Human monocytic THP-1 cells were maintained in culture in Roswell Park Memorial Institute medium (RPMI 1640, Invitrogen) culture medium containing 10 % of heat inactivated fetal bovine serum (Invitrogen) and supplemented with 10 mM Hepes (Gibco, #15630-056), 1 mM pyruvate (Gibco, #11360-039), 2.5 g/l D-glucose … how to store lithium ion batteries for winter

A lncRNA from an inflammatory bowel disease risk locus …

THP1-Dual NF-κB & IFN Reporter THP1 monocyte InvivoGen

WebHuman hepatic adenocarcinoma cell line SK-HEP-1 (ATCC, HTB-52), human breast adenocarcinoma cell line MDA-MB-231 (ATCC, HTB-26), and human lung cancer cell line … WebNov 1, 2024 · For trans-well co-culture assays, THP-1 cells (ATCC, Rockville, MD, USA) were seeded into a 24-well plate (lower chamber, 3 × 10[sup.5]) and differentiated into macrophages (M0) with 150 nM phorbol 12-myristate 13-acetate (PMA, Sigma-Aldrich) for 24 h, after which the PMA-containing medium was replaced with 10% FBS containing a 1 × … read zip file in sparkWebDec 10, 2024 · The development of immunomodulatory drugs and biologics dictates a clear demand for human cell-based models to evaluate immune activation. To address this … how to store lithium polymer batteries

"Web3- Purification of monocyte TP-1 cell line derived IDEM and encapsulation of tri-iodinated I 131biotin-PKM2[10]: Monocytic cell line (ThP-1 cells) was purchased from ATCC. Cells were grown in Roswell Park Memorial Institute (RPMI)-1640 medium (ATCC 30-2001TM) containing 10% (v/v) fetal bovine serum, 100 U/mL penicillin, 0.1 mg/mL streptomycin ... " - Thp-1 cell atcc

Thp-1 cell atcc

Rapid preparation of high-purity nuclear proteins from a small …

WebApr 13, 2024 · To obtain cell suspensions for the TPI-FC experiments, THP-1 cells were harvested from the cell culture flask, centrifuged for 5 min at 1500 rpm and resuspended in PBS solution containing 10% FBS ... WebHigh molecular weight genomic DNA isolated from the THP-1 human monocyte cell line, ATCC TIB-202. Powered by Bioz See more details on Bioz. Product category. Human …

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WebDec 10, 2024 · This webinar will present data showing that THP-1 luciferase reporter cell lines are valuable tools for studying signal transduction pathways, screening of … WebChlamydia pneumoniae disturbs cholesterol homeostasis in human THP-1 macrophages via JNK-PPARγ dependent signal transduction pathways. Author links open overlay panel Wei Liu a b 1, Ping He a 1, Bei Cheng a, Chun-Li Mei a, Yan ... induces macrophage-derived foam cell formation, a hallmark of early atherosclerosis, in the presence of low ...

WebATCC primary human CD14+ monocytes are capable of differentiating into macrophages and dendritic cells as assessed by common ... A comparison of differences in the gene …

Web1.1 材料人单核细胞(THP-1细胞)购自美国ATCC；DMSO培养基、佛波酯(PMA)购自美国 Sigma公司；FBS、EDTA购自美国Gibcog公司；吡格列酮(Piog，PPAR-γ激动剂)、T0070907(PPAR-γ抑制剂)、LY294002即蛋白激酶B(AKT)抑制剂购自美国Abmole公司；AKT抗体、p-AKT抗体购自美国Cell Signaling Technology；PPAR-γ抗体购自北京柯诺 … WebTHP-1 cells were infected with S. Typhimurium wild type or ... USA) was used, which was added for 48 hours. Human HeLa cells and RAW264.7 murine macrophages (ATCC# TIB-71, ATCC, USA) were cultured in DMEM media supplemented with 10% fetal bovine serum (FBS) and 100 μg/mL penicillin/streptomycin (Life Technologies Inc., USA).

Webresearch and development for decades. To address this need, ATCC introduced luciferase reporters containing the response element of immunologically important transcription …

WebEfficient non-viral transfection of THP-1 cells. Categories: Transfection . Authors: Schnoor M, Buers I, Sietmann A, Brodde MF, Hofnagel O, Robenek H, Lorkowski S. In: J Immunol Methods (2009) 344(2): 109-15 . Tribbles-2 is a novel regulator of inflammatory activation of … how to store lithium ion batteries long termWebHighlighted purified nuclear protein is required when using in electrophoretic mobility shift assay (EMSA) toward study transcription factors, e.g. nuclear factor-κB (NF-κB), a large copy factor so regulates both inherently and adaptive immune responses following infection. Although of protocols have come developed for nuclear eiweis extraction, people live not … read zip file and convert to byte array c#WebNov 18, 2016 · Using the in vitro (THP-1 cells) and ex vivo (primary monocytes) approach side-by-side allowed us to make informed judgments on the usefulness of THP-1 cells as a model for primary monocytes. For example, monocytes, when compared with THP-1 cells, are far more responsive to LPS. The remarkable LPS responsiveness of human peripheral … read zip files pythonWebTHP-1 cells are a human monocyte suspension cell line from peripheral blood of a 1 year old infant who had acute mnocytic leukemia. how to store lithium batteries long termWebMar 16, 2024 · (A) THP-1 cells were pretreated with different cell death inhibitors ZVAD (50 µm), Fer-1 (3 µm), Necro (3 µm), and GSK-872 (3 µm) for 1 h, followed by 1 µg/mL of E protein stimulation for 4 h. The release of LDH to the supernatant and the cell viability were detected respectively using the commercial kits. read zatch bell mangaWebMaintaining healthy cells is the key to experimental success and reproducible research results. To give you confidence in the health of your cells every step of the way, we’ve highlighted the technologies and products within cell biology that are critical to maintaining optimal cell health. No matter how you are using your cells, you can ... read zathura bookWebTHP-1 reporter cells ATCC offers luciferase reporters containing the response element of immunologically important transcription factors into the THP-1 cell line. These advanced … read �the newbie is too strong free