Hieff clone plus multi one step cloning kit
WebDescription. This kit is further developed based on the principle of efficient and rapid connection of DNA fragments by topoisomerase. Compared with the traditional T4 ligase, … Webusing Hieff Clone Plus One Step Cloning Kit (Yeasen Biotech Co. Ltd.) and the vectors were transformed into EscherichiacoliDH5 cells.Sequenceswereconfirmedby DNA sequencing. The plasmids were extracted by Plasmid Maxi Preparation Kit or Plasmid Maxiprep Kit (Bey-otime, Shanghai, China), and subsequently transfected into
Hieff clone plus multi one step cloning kit
Did you know?
Web17 de set. de 2024 · On one side, DRAM1 interacted ... BioID2 plasmid, respectively. 3FLAG-DRAM1 and GCaMP6 or DRAM1 and BioID2-FLAG were homologously recombined using the Hieff Clone® Plus Multi One Step Cloning Kit ...
WebDescription. This kit is further developed based on the principle of efficient and rapid connection of DNA fragments by topoisomerase. Compared with the traditional T4 ligase, … WebUniversal one step cloning kit, a one step cloning kit, is developed by seamless cloning technology based on homologous recombinase. Compared with the traditional enzyme …
Web1 de jul. de 2024 · Secondly, fragments of EGFP coding sequence (amplified with EGFP-ORF-F and EGFP-ORF-R) and Pxvasa 3′UTR (amplified with vasa3′UTR-F and vasa3′UTR-R) were used to construct two transient expression vectors vasaP2k-EGFP-SV40 and vasaP1214-EGFP-vasa3′UTR using Hieff Clone Plus Multi One Step Cloning Kit … WebTA Cloning kits are available without competent cells (K2024-20 and K2024-40) or with Invitrogen One Shot INVαF’ Chemically Competent E. coli (K2000-01 and K2000-40), and One Shot TOP10F’ Chemically Competent E. coli (K2040-01 and K2040-40) in both 20 and 40-reaction pack sizes.For TA Cloning with the pCR II Vector (dual promoter) kits are …
Web13 de abr. de 2024 · 高保真聚合酶（Hieff Canace Plus High-Fidelity DNA Polymerase，货号 10153ES60） 一步法克隆试剂盒（Hieff Clone ® Plus One Step Cloning Kit 一步 …
http://yeasen.com/assets/upload/file/20240817130924833.pdf bird in hand hotel lancaster pennsylvaniaWebBGC0001040: rapamycin biosynthetic gene cluster from Streptomyces rapamycinicus NRRL 5491. bird in hand ice cream shopWebElisa Kit / ... Hieff Clone ® 多片段一 ... 10912ES10. 10 T. Hieff Mut TM 定点突变试剂盒. 11003ES10. 10 T . HB211123. 推荐商品 (10911ES) Hieff Clone ® Plus One Step … damaghi first qualityWeb28 de set. de 2024 · Hieff Clone Plus Multi One Step Cloning Kit (YEASEN, Shanghai, China) was used to develop co-expressing plasmids of pET-rrbdh-nox, pET-rrbdh-nox-vgb, pET-ssbdh-nox, and pET-ssbdh-nox-vgb according to the provided protocol. damaging a wireless deviceWebBest practices. With focuses on the research and development and manufacturing of tool enzyme raw materials, antigen and antibodies, Yeasen supplies 40+ types of enzymes, … damaging chemicals in shampooWeb1 de jan. de 2024 · Gibson assembly was performed with Hieff Clone TM Plus Multi One Step Cloning Kit (Yeasen, Shanghai, China). The substrate ferulic acid was dissolved in H 2 O as stocking solution (800 mM) and the pH was adjusted to around 7.0 by NaOH. ... which was assembled with Gibson assembly kit, resulting in plasmid pLux-RFP. 2.2.6. damaging effects of marijuana on adhd brainWebGenBuilder™ Cloning Kit: GenBuilder™ Plus Cloning Kit: 1: Unique need fulfilled by the kit: Multi-gene cloning for up to 6 fragments: Multi gene cloning for up to 12 fragments: 2: Cost per reaction: $7.90: $9.80: 3: Number of fragments that can be cloned into a linearized vector: 6: 12: 4: Cloning efficiency (insert was cloned into the ... bird in hand in bird in hand